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- 1From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedTranscriptional regulation has a pivotal role in controlling gene expression, which is instrumental for organisms during development and in response to the environment. Therefore, determining how transcription factors...
- 2From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedGateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources,...
- 3From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedWe report a method for high-throughput, cost-efficient empirical discovery of optimal proteotypic peptides and fragment ions for targeted proteomics applications using in Wiro-synthesized proteins. We demonstrate the...
- 4From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedThe primary tenets of design are utility and function. Just as objects are intuitive to use when they are well-designed, thoughtfully conceived scientific figures, slides and posters can be easy to interpret and...
- 5From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedWe present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele-expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced...
- 6From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedThe precise co-localization and stoichiometric expression of two different light-gated membrane proteins can vastly improve the physiological usefulness of optogenetics for the modulation of cell excitability with...
- 7From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedTo the Editor: Mapping protein-protein interactions (PPIs) has proven instrumental in functional proteomics. Large PPI databases have been established by literature mining or using high-throughput screening methods;...
- 8From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedFluorescent proteins have become indispensable imaging tools for biomedical research. Continuing progress in fluorescence imaging, however, requires probes with additional colors and properties optimized for emerging...
- 9From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedIn malaria parasites, the systematic experimental validation of drug and vaccine targets by reverse genetics is constrained by the inefficiency of homologous recombination and by the difficulty of manipulating adenine...
- 10From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedMonya Baker Nat. Methods 8, 799-803 (2011); corrected after print 28 October 2011. In the version of this article initially published, a figure was incorrectly attributed. It is reprinted from reference 3. The...
- 11From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedGenome-wide mutagenesis in mouse embryonic stem cells (ESCs) is a powerful tool, but the diploid nature of the mammalian genome hampers its application for recessive genetic screening. We have previously reported a...
- 12From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedAccess to unlimited quantities of human heart cells for research, drug discovery and therapeutic applications is one of the most exciting promises of stem-cell research. But how do you get these specialized cells in...
- 13From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedA major challenge in systems biology is to understand the gene regulatory networks that drive development, physiology and pathology. Interactions between transcription factors and regulatory genomic regions provide the...
- 14From: Nature Methods. (Vol. 8, Issue 12) Peer-Reviewed
Training the kit generation: young scientists must learn not just how to use a kit, but how it works
Modern molecular biology would be impossible without commercial kits. Countless scientists find these prepackaged protocols indispensable for such workaday tasks as amplifying DNA, isolating plasmids, extracting RNA and... - 15From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedThree-dimensional (3d) structured-illumination microscopy (SIM) can double the lateral and axial resolution of a wide-field fluorescence microscope but has been too slow for live imaging. Here we apply 3D SIM to living...
- 16From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedThe discovery of light-gated ion channels and ion pumps in green algae and bacteria began a new era in neuroscience, now yielding a bumper crop of exciting discoveries every year. As it turns out, these microbial light...
- 17From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedErnst Bamberg has grown used to surprises from microbial rhodopsins. About a decade ago, he, together with Georg Nagel and Peter Hegemann, characterized the proteins that help green algae move toward light. Unlike...
- 18From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedDrosophila melanogaster has one of the best characterized metazoan genomes in terms of functionally annotated regulatory elements. To explore how these elements contribute to gene regulation, we need convenient tools to...
- 19From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedTo the Editor: Advances in next-generation sequencing have reshaped the landscape of genomic and epigenomic research. Large consortia such as the Encyclopedia of DNA Elements, the Roadmap Epigenomics Mapping Consortium...
- 20From: Nature Methods. (Vol. 8, Issue 12) Peer-ReviewedNKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-[5.sup.eGFP/w] hESCs facilitate quantification of cardiac differentiation,...