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- 1From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedTime-resolved, single-particle electron cryomicroscopy allows biological processes to be followed by taking multiple snapshots of dynamic systems, but a very large computational effort is required to sort images of...
- 2From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedWe have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This...
- 3From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedWe report ultrasensitive [Ca.sup.2+] indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the [Ca.sup.2+]-sensing domain of a genetically encoded [Ca.sup.2+] indicator, YC2.60 or YC3.60. Their high...
- 4From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedResearchers designed an enzyme to carry out the Diels-Alder reaction, an activity not found in nature. Enzymes have been honed over millions of years of evolution to have unique specificities for catalyzing chemical...
- 5From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedEngineered kinases remain off in cells until turned on by small molecules. The Nobel prize may have been awarded for the creation of knockout mice, but there are many questions that cannot be answered by deleting a...
- 6From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedFor several years now, Stan Fields has wanted an easy, comprehensive tool to study a common question: how a protein's function is affected by genetic variance. He and members of his laboratory at the University of...
- 7From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedRodents are frequently used to model damage and diseases of the central nervous system (CNS) that lead to functional deficits. Impaired locomotor function is currently evaluated by using scoring systems or biomechanical...
- 8From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedCaenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin...
- 9From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedThe low abundance of ubiquitination sites make them difficult to detect. Xu et al. now describe a method to enrich these previously ubiquitinated peptides with a monoclonal antibody reagent that recognizes a unique...
- 10From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedSugar-based post-translational modifications remain a challenge to characterize, owing to their low abundance and diverse chemistries. Rexach et al. now describe a straightforward approach to characterize the...
- 11From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedStrand-specific, massively parallel cDNA sequencing (RNA-seq) is a powerful tool for transcript discovery, genome annotation and expression profiling. There are multiple published methods for strand-specific RNA-seq,...
- 12From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedFat is softer than bone, and mimicking this difference with flexible substrates has been observed to influence the differentiation of adult stem cells. In this issue of Nature Methods, Chen and co-workers have made...
- 13From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedRegulation of microtubule dynamics is essential for many cell biological processes and is likely to be variable between different subcellular regions. We describe a computational approach to analyze microtubule dynamics...
- 14From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedSingle DNA molecules stretched in a nanochannel display melting patterns characteristic of their sequence. When Walter Reisner was a graduate student in Robert Austin's laboratory at Princeton University, he was...
- 15From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedWe developed an automated system, ScanLag, that measures in parallel the delay in growth (lag time) and growth rate of thousands of cells. Using ScanLag, we detected small subpopulations of bacteria with dramatically...
- 16From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedData figures or graphs are essential to life-science communication. Using these tools authors encode information that readers later decode. It is imperative that graphs are interpreted correctly. Despite the importance...
- 17From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedTo the Editor: Pyrosequencing has revolutionized microbial community analysis by allowing the simultaneous assessment of hundreds of microbial communities in multiplex with sufficient depth to resolve meaningful...
- 18From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedWe report the establishment of a library of micromolded elastomeric micropost arrays to modulate substrate rigidity independently of effects on adhesive and other material surface properties. We demonstrated that...
- 19From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedMicroRNAs may be small, but these non-coding RNAs that regulate gene expression are creating a big stir. Finding differences in the expression of microRNAs between, say, healthy and diseased cells could potentially be...
- 20From: Nature Methods. (Vol. 7, Issue 9) Peer-ReviewedMost, if not all, human mRNAs are spliced into several isoforms by heterogeneous nuclear ribonucleoprotein (hnRNP) particles that associate with the nascent transcript. Koenig et al. now precisely map binding of an...