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- 1From: Plant Methods. (Vol. 7) Peer-ReviewedBackground Targeted Induced Loci Lesions IN Genomes (TILLING) is increasingly being used to generate and identify mutations in target genes of crop genomes. TILLING populations of several thousand lines have been...
- 2From: Plant Methods. (Vol. 7) Peer-ReviewedWith the establishment of advanced technology facilities for high throughput plant phenotyping, the problem of estimating plant biomass of individual plants from their two dimensional images is becoming increasingly...
- 3From: Plant Methods. (Vol. 7) Peer-ReviewedWheat is one of the most important crops in Australia, and the identification of young plants is an important step towards developing an automated system for monitoring crop establishment and also for differentiating...
- 4From: Plant Methods. (Vol. 7) Peer-ReviewedBackground In vivo detection of protein-bound genomic regions can be achieved by combining chromatin-immunoprecipitation with next-generation sequencing technology (ChIP-seq). The large amount of sequence data...
- 5From: Plant Methods. (Vol. 7) Peer-ReviewedThe increasing popularity of systems-based approaches to plant research has resulted in a demand for high throughput (HTP) methods to be developed. RNA extraction from multiple samples in an experiment is a significant...
- 6From: Plant Methods. (Vol. 7) Peer-ReviewedBackground Cultivated sunflower (Helianthus annus L.) is a globally important oilseed crop, subjected to intensive genetic and genomic studies. Although classical mutagenesis has successfully been applied to...
- 7From: Plant Methods. (Vol. 7) Peer-ReviewedBackground The Arabidopsis thaliana-Pseudomonas syringae model pathosystem is one of the most widely used systems to understand the mechanisms of microbial pathogenesis and plant innate immunity. Several inoculation...
- 8From: Plant Methods. (Vol. 7) Peer-ReviewedBackground Many experiments in modern plant molecular biology require the processing of large numbers of samples for a variety of applications from mutant screens to the analysis of natural variants. A severe...
- 9From: Plant Methods. (Vol. 7) Peer-ReviewedBackground Histochemical staining of plant tissues with 4-dimethylaminocinnamaldehyde (DMACA) or vanillin-HCl is widely used to characterize spatial patterns of proanthocyanidin accumulation in plant tissues. These...
- 10From: Plant Methods. (Vol. 7) Peer-ReviewedA method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based...
- 11From: Plant Methods. (Vol. 7) Peer-ReviewedProteins in the plant apoplast are essential for many physiological processes. We have analysed and compared six different infiltration solutions for proteins contained in the apoplast to recognize the most suitable...
- 12From: Plant Methods. (Vol. 7) Peer-ReviewedBackground Virus-induced gene silencing (VIGS) is an effective technology for the analysis of gene functions in plants. Though there are many reports on virus vectors for VIGS in plants, no VIGS vectors available for...
- 13From: Plant Methods. (Vol. 7) Peer-ReviewedBackground Plant protoplasts, a proven physiological and versatile cell system, are widely used in high-throughput analysis and functional characterization of genes. Green protoplasts have been successfully used in...
- 14From: Plant Methods. (Vol. 7) Peer-ReviewedWe outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a mutant...
- 15From: Plant Methods. (Vol. 7) Peer-ReviewedThe evaluation of yield-related traits is an essential step in rice breeding, genetic research and functional genomics research. A new, automatic, and labor-free facility to automatically thresh rice panicles, evaluate...
- 16From: Plant Methods. (Vol. 7) Peer-ReviewedBackground The foundations for ethylene research were laid many years ago by researchers such as Lizada, Yang and Hoffman. Nowadays, most of the methods developed by them are still being used. Technological...
- 17From: Plant Methods. (Vol. 7) Peer-ReviewedBackground The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to...
- 18From: Plant Methods. (Vol. 7) Peer-ReviewedBackground Total internal reflection fluorescence microscopy (TIRFM) is a powerful tool for observing fluorescently labeled molecules on the plasma membrane surface of animal cells. However, the utility of TIRFM in...
- 19From: Plant Methods. (Vol. 7) Peer-ReviewedBackground Plant defense against herbivory has been studied primarily in aerial tissues. However, complex defense mechanisms have evolved in all parts of the plant to combat herbivore attack and these mechanisms are...
- 20From: Plant Methods. (Vol. 7) Peer-ReviewedMotivation Complete organellar genome sequences (chloroplasts and mitochondria) provide valuable resources and information for studying plant molecular ecology and evolution. As high-throughput sequencing technology...