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- 1From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedThey are the quintessential drug target--but the dynamic structures and highly elaborate mechanisms of G protein-coupled receptors continue to keep experts in both industry and academia on their toes. It is...
- 2From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedGold nanoparticles are used to monitor caspase activity at the single-molecule level in living cells. If a biologist could be a fly on the membrane, peering into the interior of the cell, watching single molecules as...
- 3From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedMicroRNAs are important regulators of gene expression, yet the functional outputs of most microRNA-target interactions remain elusive. Here we introduce the Drosophila melanogaster microRNA sponge (miR-SP) as a powerful...
- 4From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedTwo methods enable the drawing of genome-wide chromatin interaction maps: one looks at protein-independent folding principles, the other at protein-mediated functional interactions. In its stretched out form, human...
- 5From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedPavel Novak, Chao Li, Andrew I Shevchuk, Ruben Stepanyan, Matthew Caldwell, Simon Hughes, Trevor G Smart, Julia Gorelik, Victor P Ostanin, Max J Lab, Guy W J Moss, Gregory I Frolenkov, David Klenerman 8z Yuri E Korchev...
- 6From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedModifications in the cores of histones have important roles in regulating the structure and function of chromatin. Neumann et al. describe an approach to recombinantly generate site-specifically acetylated histones...
- 7From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedTo image synaptic activity within neural circuits, we tethered the genetically encoded calcium indicator (GECI) GCaMP2 to synaptic vesicles by fusion to synaptophysin. The resulting reporter, SyGCaMP2, detected the...
- 8From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedMicroRNAs regulate gene expression by binding to mRNAs and inhibiting translation. Algorithms for discovering microRNA-mRNA pairs have been developed but are prone to inaccuracies, so Ritchie et al. set out to develop...
- 9From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedTo the Editor: In their recent Perspective, Cusick et al. (l) state "... curation can be error-prone and possibly of lower quality than commonly assumed." Although we welcome rigorous scrutiny of curation efforts,...
- 10From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedThe nematode Caenorhabditis elegans has a compact nervous system with only 302 neurons. Whereas most of the synaptic connections between these neurons have been identified by electron microscopy serial reconstructions,...
- 11From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedIn the nineteenth century, light microscopy coupled with cellular labels provided insights into neuronal organization that were critical for establishing the neuron doctrine--a central tenet of modern neuroscience....
- 12From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedOptical studies have revealed that, after binding, virions move laterally on the plasma membrane, but the complexity of the cellular environment and the drawbacks of fluorescence microscopy have prevented access to the...
- 13From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedTwo improved genetically encoded calcium indicators--based on structure--guided sensor design or on precise subcellular targeting to presynaptic boutons--allow single spikes to be detected in genetically defined...
- 14From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedNew chemical microarrays capture a comprehensive snapshot of the various enzymatic activities contained within a biological sample. Even as next-generation sequencing platforms have delivered rapid and accurate...
- 15From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedMatija Dreze, Benoit Charloteaux, Stuart Milstein, Pierre-Olivier Vidalain, Muhammed A Yildirim, Quan Zhong, Nenad Svrzikapa, Viviana Romero, Geraldine Laloux, Robert Brasseur, Jean Vandenhaute, Mike Boxem, Michael E...
- 16From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedMicroRNA depletion by sequestration produces flexible hypomorphs that mimic mutants obtained by microRNA gene targeting. MicroRNAs (miRNAs) are relatively new on the scene as regulators of gene expression. Their...
- 17From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedTo the Editor: GFP, its homologs and many mutants have become extremely popular for the labeling of living cells in fluorescence microscopy (1). Recently, we discovered that excited green fluorescent proteins can donate...
- 18From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedZebrafish embryos offer a unique combination of high-throughput capabilities and the complexity of the vertebrate animal for a variety of phenotypic screening applications. However, there is a need for automation of...
- 19From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedPlants have evolved a unique system in which the plant hormone auxin directly induces rapid degradation of the AUX/IAA family of transcription repressors by a specific form of the SCF E3 ubiquitin ligase. Other...
- 20From: Nature Methods. (Vol. 6, Issue 12) Peer-ReviewedAn imaging platform based on stimulated emission helps researchers to lead nonfluorescent chromophores out of the shadows. For years now, fluorescent reagents and proteins have reigned as the undisputed champions of...