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- 1From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedAlthough microRNA target predictions are continually improving, high-throughput validation of direct interaction is still needed. The small but biologically extremely influential microRNAs were again under intense...
- 2From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedScientists create a high-throughput platform for proteome-scale assessment of protein stability. Proteins are the work horses of the cell, and fluctuations in their expression are fundamental for metabolism, cell-cycle...
- 3From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedMethods to reprogram somatic cells to pluripotency have improved and will improve further; more biological studies of these cells are forthcoming. When, a year ago, we picked induced pluripotent (iPS) stem cells as an...
- 4From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedA device to cool localized areas of the zebra finch brain allows researchers to investigate how the timing of birdsong is controlled. The zebra finch sings a complex song made up of repeating elements called motifs,...
- 5From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedWe developed infrared laser-evoked gene operator (IR-LEGO), a microscope system optimized for heating cells without photochemical damage. Infrared irradiation causes reproducible temperature shifts of the in vitro...
- 6From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedUntil not very long ago, it was widely accepted that lens-based (far-field) optical microscopes cannot visualize details much finer than about half the wavelength of light. The advent of viable physical concepts for...
- 7From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedA new spin on a popular imaging technique allows researchers to accurately visualize tumors deep within the tissues of live mice. In principle, the dizzying array of fluorescent probes currently available for...
- 8From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedOur variant ascertainment algorithm, VAAL, uses massively parallel DNA sequence data to identify differences between bacterial genomes with high sensitivity and specificity. VAAL detected ~98% of differences (including...
- 9From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedDirected evolution via phage display is a well-established method to generate proteins with new or improved activities. Liu et al. now present a phage-display directed-evolution system with an expanded genetic code, by...
- 10From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedTagging ribosomes in a cell type-specific way allows the isolation of mRNAs that are being translated in these cells. "If the human brain were so simple that we could understand it, we would be so simple that we...
- 11From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedNew methods addressing the challenges in membrane protein expression, solubilization and crystallization promise to yield many more atomic structures. Membrane proteins make up roughly 30% of the proteome, but they are...
- 12From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedComprehensive protein-interaction mapping projects are underway for many model species and humans. A key step in these projects is estimating the time, cost and personnel required for obtaining an accurate and complete...
- 13From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedIn December 2006 we published a special issue dedicated to protein-protein interaction (PPI) methods. Now almost exactly two years later, we again present a collection of research papers on this subject. The differences...
- 14From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedInformation on protein-protein interactions is of central importance for many areas of biomedical research. At present no method exists to systematically and experimentally assess the quality of individual interactions...
- 15From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedQuantitative mass spectrometry-based proteomics is now being applied on a large scale to address interesting biological questions. Mass spectrometry has made protein identification fairly routine and is now widely used...
- 16From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedLight microscopy has been a favorite tool of biologists for unlocking life's mysteries since Leeuwenhoek first focused light through a lens to study living microorganisms, or "animalcules," in the seventeenth century....
- 17From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedIn his 1935 book, The Design of Experiments (1), British mathematician Ronald A. Fisher developed a mathematical framework for designing experiments. Fisher explored how experiments could be most efficiently set up to...
- 18From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedFluorescence imaging 101 When fluorescent molecules--fluorophores--absorb a photon, they almost always emit a photon of longer-wavelength light about one nanosecond later. Modern detection devices such as...
- 19From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedStudying microRNA function in vivo requires genetic strategies to generate loss-of-function phenotypes. We used lentiviral vectors to stably and specifically knock down microRNA by overexpressing microRNA target...
- 20From: Nature Methods. (Vol. 6, Issue 1) Peer-ReviewedMicroRNAs (miRNAs) are a chnologies to study the unique properties of miRNAs are still needed. In this issue Luigi Naldini and colleagues present techniques to manipulate miRNA expression by expressing decoy miRNA...