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- 1From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedThis protocol describes the aerobic oxidation of aromatic anilines to aromatic azo compounds using gold (Au) nanoparticles supported on Ti[O.sub.2] as a catalyst. Yields above 98% are achieved under a few bars of oxygen...
- 2From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedTransplantable tumors are an accepted gold standard in cancer studies in rodents. The progress of this model in zebrafish has long been constrained by the lack of true inbred lines in zebrafish. We have generated...
- 3From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedUnlike humans, mouse bone marrow-derived mesenchymal stem cells (MSCs) cannot be easily harvested by adherence to plastic owing to the contamination of cultures by hematopoietic cells. The design of the protocol...
- 4From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedSegmental isotopic labeling is a powerful labeling technique for reducing nuclear magnetic resonance (NMR) signal overlap, which is associated with larger proteins by incorporating stable isotopes into only one region...
- 5From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedElucidation of the neural substrates underlying complex animal behaviors depends on precise activity control tools, as well as compatible readout methods. Recent developments in optogenetics have addressed this need,...
- 6From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedHere we describe a simple and efficient human embryonic stem (ES) and induced pluripotent stem (iPS) cells cryopreservation protocol. This protocol involves the use of Rho-associated kinase (ROCK) inhibitor, Y-27632,...
- 7From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedMarine-derived fungi have been shown in recent years to produce a plethora of new bioactive secondary metabolites, some of them featuring new carbon frameworks hitherto unprecedented in nature. These compounds are of...
- 8From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedNuclear magnetic resonance (NMR)-based metabolomics has many applications in plant science. Metabolomics can be used in functional genomics and to differentiate plants from different origin, or after different...
- 9From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedAccurate diagnosis of parasitic infections is of pivotal importance for both individual patient management and population-based studies, such as drug efficacy trials and surveillance of parasitic disease control and...
- 10From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedRecombinant rabies viruses rendered replication-deficient by the deletion of their envelope glycoprotein gene are useful tools for neuroscientists, permitting (1) extraordinarily high transgene expression levels within...
- 11From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedReprogramming of somatic cells into pluripotent stem cells has been reported by introducing a combination of several transcription factors (Oct3/4, Sox2, Klf4 and c-Myc). The induced pluripotent stem (iPS) cells from...
- 12From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedWe describe here a protocol for digital transcriptome analysis in a single mouse oocyte and blastomere using a deepsequencing approach. In this method, individual cells are isolated and transferred into lysate buffer by...
- 13From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedFemtosecond laser microsurgery is a powerful method for studying cellular function, neural circuits, neuronal injury and neuronal regeneration because of its capability to selectively ablate sub-micron targets in vitro...
- 14From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedMethods development in chromatography is a time-consuming, trial-and-error process that requires laborious experimentation. We describe a high-throughput screening (HTs) protocol for the rapid identification of...
- 15From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedChromatin immunoprecipitation (ChIP) is a powerful technique to study interactions between transcription factors (TFs) and DNA in vivo. For genome-wide de novo discovery of TF-binding sites, the Dna that is obtained in...
- 16From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedThis protocol is for the ultrasound (US)-assisted 1,3-dipolar cycloaddition reaction of azides and alkynes using metallic copper (Cu) as the catalyst. The azido group is a willing participant in this kind of organic...
- 17From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedA protocol for separation of positionally isomeric trans- and cis-octadecenoic fatty acids as their p-methoxyphenacyl esters, using a single column silver ion high-performance liquid chromatography (Ag-HPLC) with UV...
- 18From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedThis protocol provides an introduction to soft lithography--a collection of techniques based on printing, molding and embossing with an elastomeric stamp. soft lithography provides access to three-dimensional and curved...
- 19From: Nature Protocols. (Vol. 5, Issue 3) Peer-ReviewedG protein-coupled receptors (GPCRs) and their downstream signaling cascades contribute to most physiological processes and a variety of human diseases. Isolating the effects of GPCR activation in an in vivo experimental...