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- 1From: Nature Protocols. (Vol. 4, Issue 7) Peer-ReviewedThis protocol describes the purification of a recombinant high molecular weight (HMW) two-partner secretion exoprotein (generically referred to as TpsA proteins) from Escherichia coli using methods developed recently to...
- 2From: Nature Protocols. (Vol. 4, Issue 7) Peer-ReviewedThe elucidation of protein-protein interaction networks is a crucial task in the postgenomic era. In this protocol, we describe our approach to discover protein-protein interactions using the surface plasmon resonance...
- 3From: Nature Protocols. (Vol. 4, Issue 7) Peer-ReviewedWe present a method for the computational reconstruction of the 3-D morphology of biological objects, such as cells, cell conjugates or 3-D arrangements of tissue structures, using data from high-resolution microscopy...
- 4From: Nature Protocols. (Vol. 4, Issue 7) Peer-ReviewedThe development of animal models of lung cancer is critical to our understanding and treatment of the human disease. Conditional mouse models provide new opportunities for testing novel chemopreventatives, therapeutics...
- 5From: Nature Protocols. (Vol. 4, Issue 7) Peer-ReviewedThe effect of genetic mutation on phenotype is of significant interest in genetics. The type of genetic mutation that causes a single amino acid substitution (AAS) in a protein sequence is called a non-synonymous single...
- 6From: Nature Protocols. (Vol. 4, Issue 7) Peer-ReviewedThis protocol describes a technique for picking individual nannofossils for geochemical analysis. The protocol allows for preparation of both individual nannofossil specimens and monospecific populations for analysis. A...
- 7From: Nature Protocols. (Vol. 4, Issue 7) Peer-ReviewedThe analysis of highly hydrophobic proteins is still an analytical challenge. Using a recombinant gamma-aminobutyric acid A ([GABA.sub.A])-receptor subunit as a model protein, we developed a gel-based proteomic approach...
- 8From: Nature Protocols. (Vol. 4, Issue 7) Peer-ReviewedViruses are the most abundant and dynamic biological entities in the world's ecosystems. Marine sediments, the largest biome in the world, have the potential to represent an optimal environment for viral development. To...
- 9From: Nature Protocols. (Vol. 4, Issue 7) Peer-ReviewedTransient interactions among glycoconjugates underlie developmental, immunological and metastatic recognition. Glycan-mediated interactions have low binding affinities and rapid dissociation rates. As a result, these...