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- 1From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedExtractive electrospray ionization mass spectrometry (EESI-MS) allows the real-time, direct analysis of complex gaseous and liquid samples without any sample pretreatment under ambient conditions. By using a neutral...
- 2From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedWe have developed protocols for rapidly quantifying the band intensities from nucleic acid chemical mapping gels at single-nucleotide resolution. These protocols are implemented in the software SAFA (semi-automated...
- 3From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedVentricular pressure-volume relationships have become well established as the most rigorous and comprehensive ways to assess intact heart function. Thanks to advances in miniature sensor technology, this approach has...
- 4From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedRealizing the potential of human embryonic stem cells (hESCs) in research and commercial applications requires generic protocols for culture, expansion and genetic modification that function between multiple lines. Here...
- 5From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedA protocol for measuring the total plasma homocysteine (tHCY) concentration in very small samples using a selective, recombinant homocysteine α, γ-lyase (rHCYase) and a small portable fluorescence reader is described....
- 6From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedThe membrane-permeant fluorogenic biarsenicals FlAsH-[EDT.sub.2] and ReAsH-[EDT.sub.2] can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin,...
- 7From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedA fundamental question in olfaction is which odorant receptors (ORs) are activated by a given odorant. A major roadblock to investigating odorant-OR relationships in mammals has been the inability to express ORs in...
- 8From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedThis protocol details a procedure, known as the modified preplate technique, which is currently used in our laboratory to isolate muscle cells on the basis of selective adhesion to collagen-coated tissue culture plates....
- 9From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedPCR-based gene-targeting approaches have increased the speed of gene function analyses in ascomycetous fungi, for example, in the diploid human fungal pathogen Candida albicans. Here we describe a protocol that utilizes...
- 10From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedThis protocol describes an efficient method for the preparation of riboswitch domains comprising up to ~200 nt containing site-specific nucleoside modifications. The strategy is based on enzymatic ligation of chemically...
- 11From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedWe describe a matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS)-based assay for human papillomavirus (HPV) genotyping--the restriction fragment mass polymorphism (RFMP) assay,...
- 12From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedTouchdown (TD) PCR offers a simple and rapid means to optimize PCRs, increasing specificity, sensitivity and yield, without the need for lengthy optimizations and/or the redesigning of primers. TD-PCR employs an initial...
- 13From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedPrimate nonhuman and human embryonic stem (ES) cells provide a powerful model of early cardiogenesis. Furthermore, engineering of cardiac progenitors or cardiomyocytes from ES cells offers a tool for drug screening in...
- 14From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedMass spectrometry (MS)-based shotgun proteomics allows protein identifications even in complex biological samples. Protein abundances can then be estimated from the counts of tandem MS (MS/MS) spectra attributable to...
- 15From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedHere we describe the generation of a double-fluorescent Cre/loxP indicator system. This protocol involves (i) all cloning steps to generate the plasmid vector (3-5 months); (ii) a guide to prepare high-efficiency...
- 16From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedThe incidence of fungal infections has increased significantly over the past decades. Very often these infections are associated with biofilm formation on implanted biomaterials and/or host surfaces. This has important...
- 17From: Nature Protocols. (Vol. 3, Issue 9) Peer-ReviewedProtein phosphorylation and dephosphorylation play an important role in regulation of intracellular signal transduction pathways in the biological system. A key step in the biological characterization of phosphatases and...